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PP Systems CIRAS-3 User Manual
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CIRAS-3
Porta
ble Ph
otosy
nthesi
s Syst
em
Operati
on Manual
Vers
ion 1
.09
2017 PP S
ystem
s. All Rig
hts R
eserved
7
th
August 2017
PP System
s
110 Haver
hill Roa
d, Su
ite 301
Ames
bury, MA 01
913 U.
S.A.
Tel: +1 978
-
834
-
0
505 Fax
: +1 978
-
834
-
0
545
Em
ail:
supp
ort@pps
ystem
s.com
URL:
www.pps
y
s
tems.c
om
2
Table of Contents
Default Chapter
2
Table of Contents
2
Welcome
10
User Registration
11
Service & Warranty
12
Returning Equipment to PP Systems
12
Contact Information
13
Unpacking and Storage of Your Equipment
14
Unpacking
14
Storage
14
CIRAS-3 Tutorial & Basic Operation
15
Let's Get Started
16
Charge Internal Battery Packs
16
Inspect Desiccants
16
Insert a Fresh CO 2 Cartridge
17
Connect the PLC3 Universal Leaf Cuvette to the CIRAS-3 Console
18
Connect Light Unit to PLC3 Universal Leaf Cuvette
18
Power up the CIRAS-3 System
19
System Warm up
19
Setting up the CIRAS-3 System
20
Setting up the Graphical Display for Data Plots
21
Let's Check out both the Numerical and Graphical Display
21
Let's Have a Look at the Environmental Controls
23
It's about Time We Start Taking some Data on a Real Plant
25
Placing the Leaf Inside the Cuvette
27
What Should I be Looking for at this Point
27
Quick Start
31
Section 1. Technical Specification
33
Section 2. What's New with CIRAS-3
36
General Changes to CIRAS-3 Hardware
36
Changes to CIRAS-3 Software
38
Section 3. Summary of System Design
40
Overview and Theory
40
Section 4. System Components and Assembly
44
CIRAS-3 Main Console
44
System Power
44
Power Supply Adapter
44
Battery Pack
45
Battery Pack Installation
46
Charging Batteries Inside the CIRAS-3 Console
47
CIRAS-3 Rear Console
48
USB Flash Drive Ports
48
Battery Charging Socket (EXT PWR) and LED
49
Power
49
Mini-USB (PC)
49
PLC Connections
49
CO 2 Cartridge Holder
49
CO 2 Cartridges
50
How Do I Determine the State of the CO Cartridge
50
Gas Ports and Link Pipe
51
Auxiliary Connector
51
Zero Column
51
CO 2 /H 2 O Control Columns and H
52
Equilibrator
52
PLC3 Series Leaf Cuvettes
53
PLC3 Universal Leaf Cuvette
53
PLC3 Narrow Leaf Cuvette
53
PLC3 Conifer Leaf Cuvette
53
PLC3 Temperature Control
54
Temperature Measurement
54
Temperature Control Type
54
LCD on Leaf Cuvette
55
PAR Measurement
56
Light Control (Optional)
57
PLC3 Universal LED Light Unit (RGBW)
57
PLC3 Narrow and Conifer LED Light Unit
58
Chlorophyll Fluorescence Module
59
Section 5. CIRAS-3 Console Software
60
Overview
60
Basics of Navigating Console Screens and Menus
61
Numeric View of Data and Control Variables
62
Section 6. Startup Routine and System Stability Checks
66
Before You Power up CIRAS-3
66
Startup and Warm up
67
CO 2 Cartridge Status
68
Changing a CO 2 Cartridge
70
Checking Stability - before You Place a Leaf in the Chamber
70
Survey Measurements - some Tips
71
Additional Filtering in Dirty/Dusty Environments
72
Section 7. Basic Functions - Viewing and Controlling CIRAS-3
73
Settings (F2)
73
Settings Graph Set (F5)
78
Controls (F3)
79
Controls Graph Set (F5)
80
Toggle View (F4)
80
Reviewing and Transferring Data Files
82
Transfer Data (F6)
82
Section 8. Operations
84
Operations (F1)
84
Recording Options
84
Manual Recording
84
Timed Recording
86
Response Curves
87
Z-Diff Bal (F7)
94
Set Clock
95
View Saved (F4)
96
Calibration
98
Recalibrate
98
Calibration Setup
99
CO 2 Calibration
99
H 2 O Calibration
100
Find Max C
101
Store Diff Bal
102
LED Calibrate
105
PAR Calibrate
106
Diagnostics
107
Irga (F2)
107
Plc (F3)
108
Flow (F4)
110
Power (F5)
111
Apa (F6)
112
Report (F7)
113
Firmware Upgrade
114
Section 9. CIRAS-3 Console and PC Utility Software
116
CIRAS-3 Console Software
116
Installation Instructions for Main CIRAS-3 Console
116
Uninstall Instructions
116
PC Utility Software
117
Installation Instructions for PC Utility
117
Uninstall Instructions
117
PC Utility Operation
118
Create/Edit Response Scripts
119
Create/Edit Settings Files
121
Remote Display
123
Section 10. Measuring Chlorophyll Fluorescence with CFM-3
127
Overview of Chlorophyll Fluorescence
127
The Basics
127
Calculations Used for Chlorophyll Fluorescence Parameters
129
Chlorophyll Fluorescence Measurements - Setup and Operation
130
CFM-3 Set up
131
CFM-3 Settings
132
CFM-3 Controls
135
Measurement of Fm' with CIRAS-3
137
Section 11. Closed System Measurements
140
Soil CO Efflux and Net Canopy CO Flux
140
Theory
140
FCO Units for Measurement of Soil CO Efflux
141
References
142
Electrical and Pneumatic Connection between the CIRAS-3 and SRC-1 / CPY-4
142
Electrical and Pneumatic Connection between the CIRAS-3 and SRC-2 / CPY-5
144
Configuring the CIRAS-3 Console for Measurement of Soil Respiration or Canopy Assimilation
144
Settings (F2)
145
Do I Use Linear Versus Quadratic
147
Closed System Operation
147
Measurement Sequence
150
Initial Test Measurement Sequence
151
Recording Measurements
151
Section 12. Data Output
153
Photosynthesis Data
153
Photosynthesis and Chlorophyll Fluorescence Data
155
Closed System Operation Data
158
Summary of the Gas Exchange Equations Used in CIRAS-3
159
Mass Flow Equation
159
Transpiration
159
Leaf Temperature
160
Saturation Vapor Pressure
161
Stomatal Conductance
161
Net Photosynthesis
161
Intercellular CO 2 Concentration
162
Physical Constants Used in Equations
163
Definition of Symbols
163
Section 13. Troubleshooting & Diagnosis
165
System Power
165
CO 2 Measurement and Control
166
H 2 O Measurement and Control
168
Temperature Measurement and Control
169
PAR Measurement and Control
170
Flow Control
171
Data Management
172
Status Codes Displayed on the CIRAS-3 LCD
173
Other Status Codes/Messages
177
Section 14. Routine Maintenance
178
CIRAS-3 Main Console
178
Battery Pack (Li-Ion Battery)
178
Battery Removal
179
Battery Storage
179
Battery Disposal
179
External Battery Pack (for Extended Operation in the Field)
180
Air Sampling Pumps (Reference and Analysis)
180
Replacement of Reference and Analysis Pump
181
Servicing the Air Sampling Pump (Reference & Analysis)
181
Air Supply Pump
182
Air Filters
183
External Air Inlet Filter Assembly (Part Number STD558)
184
Desiccants and Absorber Columns
184
Zero Column Assembly
185
CO 2 & H 2 O Control Column
186
COO Control Column
186
Soda Lime
187
Drierite
187
Molecular Sieve
188
Molecular Sieve Repackaging
189
Foam Filters
189
Absorber Filters
190
O" Rings
190
CO 2 Regulator
190
CO 2 Calibration
192
H 2 O Calibration
193
PLC3 Leaf Cuvettes
193
Cuvette Gaskets
193
Checking for Leaks Associated with the PLC3
194
PLC3 Leaf Cuvette Head Adjustment
195
PLC3 Pneumatic Connector
196
Environmental Sensors
196
Temperature
196
Par
196
Boundary Layer Determination (R B )
197
Changing PLC3 Universal Head Plates
200
Replacement of PLC3 Glass Window (for Use with CFM-3)
201
Section 15. Consumables/Spares
202
Desiccants and Absorber Columns
202
Pumps & Air Filters
202
CO 2 /H 2 O Control
203
COO Control
203
Batteries & Power Supplies
203
Cables/Data Storage
203
Leaf Cuvette Accessories
203
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PP Systems CIRAS-3 Specifications
General
Brand
PP Systems
Model
CIRAS-3
Category
Measuring Instruments
Language
English
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