32 BioPAT
®
Trace | Multi Trace Operating Instructions
Design and Function
For the dialysis method, the internal diffusion module is used to calibrate the
BioPAT
®
Trace | Multi Trace. By contrast, the dialysis probe in the bioreactor is
always used for measuring the sample. A reference factor between the two
membranes is then determined once.
As a standard cannot be installed in the bioreactor, the BioPAT
®
Trace | Multi
Trace must be calibrated indirectly by way of the reference factor. During this
process, the initial concentration in the bioreactor is used as the starting con-
centration for the dialysis probe. Using the sample measurement values with
an identical concentration, the BioPAT
®
Trace | Multi Trace calculates the fac-
tor between the diffusion module membrane and the dialysis probe
membrane.
Hence, the calibration procedure for the dialysis method consists of three
steps in total:
Step 1
Recording the calibration curves on the diffusion module with standards 1
and 2.
Step 2
Measuring the external sample on the BioPAT
®
Trace | Multi Trace with the
help of the calibration curves (alternatively: by directly entering the concen-
tration values, if known from the initial weight or by external analysis).
Step 3
Recording the calibration curves of the dialysis probe using the probe.
After measuring the standards for calculating the calibration curves, the
BioPAT
®
Trace | Multi Trace computes a factor from the gradients of each
individual curve. For all measurements carried out with the dialysis probe, this
factor is applied to calculate the true concentration.
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