4.4 Septum
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If you are using capillary column
Septum conditioning
In high sensitivity analysis, impurities from septum may be detected as ghost peaks. In that case, condition
the septum as described below.
(1)
Soak the septum in hexane for 10 to 15 hours.
When absorbing hexane the septum swells to approximately twice its original volume. Use a wide
opening container with lid.
(2)
Take out the septum and put it in a clean container.
Handle with care. The septum swollen with hexane can crumble easily.
(3)
Let the septum dry in a clean atmosphere.
(4)
After dried, bake the septum at 130 to 150C for approximately 2 hours.
Keep the conditioned septum in a sealed container. If it is left for a while after baking, it can be stained
again by absorbing impurities in the air.
Inspection procedures
First, remove the septum nut which covers the septum.
Then take out the septum, and condition or replace it.
Septum installation
Tighten the septum nut by hand, until it touches the glass insert nut below, and then loosen one half turn.
In case of manual injection, you can lengthen the septum life by tightening the septum nut by one thread
every 10 injections.
The septum nut is extremely hot when injection port is at high temperature. When tightening, ALWAYS
wear gloves, etc., to avoid burns.
Septum nut
Glass insert nut
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