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4.9 Capillary column
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4.9 Capillary column
Conditioning capillary column
To condition capillary columns, use the following procedures.
(1)
Carrier gas setting
Apply the analysis flow rate. However, by lowering the split ratio, you can conserve carrier gas.
(2)
Column oven temperature setting
Specify the temperature approx. 30C higher than the column operation temperature for analysis. In
this case, NEVER specify higher than the maximum column temperature limit. Also, NEVER raise the
temperature rapidly. (Particularly, in case of polar column)
(3)
Injection port and detector temperature setting
For injection port, you do not need to change from the temperature setting at analysis.
For detector temperature, specify approx. 30C higher than the column oven temperature.
(4)
Conditioning time
Normally, condition for 2 to 3 hours.
If the column is extremely stained, perform conditioning for longer time.
Depending on the type of column liquid phases, some columns are very fragile.
Specify conditioning temperature after verifying the operating temperature limit of your columns.
Conditioning at a temperature which is too high may break your columns.
Also, if oxygen concentration in carrier gas is high, it will greatly damage the column liquid phase. The
use of the oxygen trap is highly recommended.
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