Shimadzu LC-10/20 System 4 Using the control module
4.3.4 Method Setup - AS - Pretreatment
The logic for all calculations of pretreatment vials goes as follows: The vial
specified in the Sequence window for the injection (called the Sample
vial , SV) needs to be empty when performing pretreatment. The actual
sample is in the Source Vial (specified on the Method Setup - AS -
Pretreatment tab), while the diluent is placed in the Diluent Vial and
reagents are placed in Reagent Vials 1..3.
When using the Auto calculation of the Source Vial, the source vials are
found on the second half of the vial tray; the first half of the tray is reserved
for the empty vials used for mixing (diluting). These vials are specified in
the Sequence window and from these vials the injection is performed.
Note: In case of racks with odd number of vials, the middle vial cannot be used
as Sample Vial with pretreatment operation as it would have no
corresponding Source Vial.
Fig 39: Method Setup - AS - Pretreatment (Dilution)
Mode
Selects the pretreatment mode. There are 3 types of pretreatment
available:
Standard - Standard type of autosampler operation with no special
pretreatment. No other parameter fields are available on the Pretreatment
tab. This mode is also used when the Pretreatment tab is unavailable.
Dilution - Sample will be diluted before the injection.
Reagent - Sample will be mixed with the reagent and then injected.
Inner Needle Wash - Sampler inner needle will be washed prior and/or
after the injection.
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