4 Using the control module Clarity Controls
4.1.2 Method Setup - LC Gradient
The Method Setup - LC Gradient dialog serves for setting up the LC
instrument method.
Fig 10: Method Setup - LC Gradient
Gradient Table
A table for setting the composition of the mobile phase and the overall
flow rate as a function of time. Operation is analogous to that of
spreadsheets (Excel, Quatro Pro, etc.). To prepare the cell to receive
values, click it by the left mouse button; the cell will highlight by dots. A cell
that fails to highlight is not available for editing.
Time [min.]
Sets the time at which the ratio of flow rates and the overall flow rate
correspond to the values entered in the corresponding row. (These
values vary continuously from one time to the next in a manner
ensuring that the conditions specified in the next row are satisfied).
XXX1 (..4) [%]
Represents the percentage of a component. The designation XXX1-4
is in fact replaced by the name of the component (items Solvent 1 - 4
in the Gradient Options dialog). Should you enter a component value
such that the sum of all values exceeds 100 %, the percentage in the
last column is automatically adjusted; if the percentage of the last
compound is already zero, the value of the currently entered
component is adjusted instead. The flow rate of a compound is
calculated by multiplying the overall flow rate (indicated in the Flow
column) by the corresponding percentage divided by 100.
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