Measure OD600
132 NanoDrop One User Guide Thermo Scientific
Related Topics
• Measure a Micro-Volume Sample
• Measure a Sample Using a Cuvette
• Prepare Samples and Blanks
• Basic Instrument Operations
To measure an OD600 sample
1. From the Home screen, select the OD600 tab and tap OD600.
2. Specify the cell number conversion factor and a second monitored wavelength or
absorbance correction if desired.
3. Pipette 2 μL blanking solution (i.e., the media solution the cells of interest are
suspended in) onto the lower pedestal and lower the arm, or insert the blanking cuvette
into the cuvette holder.
Tip: If using a cuvette, make sure to align the cuvette light path with the instrument
light path.
4. Tap Blank and wait for the measurement to complete.
Tip: If Auto-Blank is On, the blank measurement starts automatically after you lower
the arm. (This option is not available for cuvette measurements.)
5. Lift the arm and clean both pedestals with a new laboratory wipe, or remove the
blanking cuvette.
6. Pipette 2 μL sample solution onto the pedestal and lower the arm, or insert the sample
cuvette into the cuvette holder.
7. Start the sample measurement:
–Pedestal: If Auto-Measure is On, lower arm; if Auto-Measure is off, lower arm and
tap Measure.
– Cuvette: Tap Measure
When the sample measurement is completed, the spectrum and reported values are
displayed (see the next section).
8. When you are finished measuring samples, tap End Experiment.
9. Lift the arm and clean both pedestals with a new wipe, or remove the sample cuvette.
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