Measure dsDNA, ssDNA or RNA
14 NanoDrop One User Guide Thermo Scientific
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the instrument
arm and clean the upper and lower pedestals. At a minimum, wipe the pedestals with a new
laboratory wipe. For more information, see Cleaning the Pedestals.
To measure nucleic acid
1. From the Home screen, select the Nucleic Acids tab
and tap dsDNA, ssDNA or RNA, depending on the
samples to be measured.
2. Specify a baseline correction if desired.
3. Pipette 1–2 μL blanking solution onto the lower
pedestal and lower the arm, or insert the blanking
cuvette into the cuvette holder.
Tip: If using a cuvette, make sure to align the cuvette
light path with the instrument light path.
4. Tap Blank and wait for the measurement to
complete.
Tip: If Auto-Blank is On, the blank measurement
starts automatically after you lower the arm. (This
option is not available for cuvette measurements.)
5. Lift the arm and clean both pedestals with a new
laboratory wipe, or remove the blanking cuvette.
6. Pipette 1-2 μL sample solution onto the pedestal and
lower the arm, or insert the sample cuvette into the
cuvette holder.
7. Start the sample measurement:
–Pedestal: If Auto-Measure is On, lower arm; if
Auto-Measure is off, lower arm and tap Measure.
– Cuvette: Tap Measure.
When the sample measurement is completed, the
spectrum and reported values are displayed (see the
next section).
8. When you are finished measuring samples, tap
End Experiment.
9. Lift the arm and clean both pedestals with a new
wipe, or remove the sample cuvette.
Typical nucleic acid spectrum
Comparison of nucleic acid spectra with
and without two common contaminants
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