Measure Protein A205
80 NanoDrop One User Guide Thermo Scientific
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the instrument
arm and clean the upper and lower pedestals. At a minimum, wipe the pedestals with a new
laboratory wipe. For more information, see Cleaning the Pedestals.
Related Topics
• Best Practices for Protein Measurements
• Measure a Micro-Volume Sample
• Measure a Sample Using a Cuvette
To measure a Protein A205 sample
1. From the Home screen, select the Proteins tab and then tap Protein A205.
2. Specify a sample type and baseline correction if desired.
3. Pipette 1–2 μL of the blanking solution onto the lower pedestal and lower the arm, or
insert the blanking cuvette into the cuvette holder.
Tip: If using a cuvette, make sure to align the cuvette light path with the instrument
light path.
4. Tap Blank and wait for the measurement to complete.
Tip: If Auto-Blank is On, the blank measurement starts automatically after you lower
the arm. (This option is not available for cuvette measurements.)
5. Lift the arm and clean both pedestals with a new laboratory wipe, or remove the
blanking cuvette.
6. Pipette 2 μL sample solution onto the pedestal and lower the arm, or insert the sample
cuvette into the cuvette holder.
7. Start the sample measurement:
–Pedestal: If Auto-Measure is On, lower arm; if Auto-Measure is off, lower arm and
tap Measure.
– Cuvette: Tap Measure
When the sample measurement is completed, the spectrum and reported values are
displayed (see the next section).
8. When you are finished measuring samples, tap End Experiment.
9. Lift the arm and clean both pedestals with a new wipe, or remove the sample cuvette.
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