Zeiss Axio Imager - Page 146

OPERATION
Carl Zeiss Illumination and contrast methods Axio Imager
146 430000-7344-001 M70-2-0020 e 06/2009
Fig. 4-114 Microscope settings on Axio Imager for transmitted-light brightfield
• Use both centering screws (4-114/6 or 4-115/1) on the condenser carrier to center the luminous-field
diaphragm image (
4-114/C). Then, open the diaphragm until its edge just disappears from the field of
view (
4-114/D).
When changing the condenser, normally the luminous-field diaphragm remains centered, unless
the centering screws were turned.
• To adjust the aperture diaphragm (contrast), remove one eyepiece from the tube and look through the
tube with your naked eye. Adjust the aperture diaphragm (
4-114/8) to approx. 2/3 ... 4/5 of the
diameter of the exit pupils of the objectives (
4-114/E). In most applications, this aperture diaphragm
setting provides optimum contrast at almost ideal resolution, and is therefore the best compromise for
the human eye.
• Insert the eyepiece back in the tube socket.