OPERATION
Axio Imager Illumination and contrast methods Carl Zeiss
M70-2-0020 e 06/2009 430000-7344-001 149
• Reinsert the eyepiece in the tube.
• If the height of the dark-field condenser is set
correctly and sensitively, it is possible to reduce
any brightening in the field of view left, and the
luminous-field diaphragm image appears almost
perfectly in focus.
• Finally, match the size of the luminous-field
diaphragm to the size of the field of view.
Dark field microscopy requires specimens to be considerably cleaner than in other techniques.
Fingerprints, dirt or dust particles, in particular, have negative effects, since they brighten the
background of the field of view and decrease the contrast of the object image.
Fig. 4-116 Centering the darkfield stop on the
universal condenser
To Next Page
Loading...
