OPERATION
Carl Zeiss Illumination and contrast methods Axio Imager
150 430000-7344-001 M70-2-0020 e 06/2009
(4) Use of the achromatic-aplanatic universal condenser 0.9 H/0.8-0.9 DF
The achromatic-aplanatic universal condenser 0.9 H/0.8-0.9 DF (
4-117/1) is equipped with a front lens
each for brightfield (
4-117/3) and for darkfield (4-117/2), as well as with an aperture diaphragm
(
4-117/6). For brightfield applications, refer to Section 4.9.1.
The requirements for the use of the universal condenser dependent on the objective used are given in the
table below.
Magnification Brightfield applications Darkfield applications
2.5x … 5x Without brightfield front lens H Without darkfield front lens D,
with wide-field DF slider for 2.5x-5x
10x … 40x With brightfield front lens H With darkfield front lens D
40 x … 100x With brightfield front lens H With darkfield front lens D,
with darkfield attachment 1.2-1.4 Oil,
Objective with adjustable iris stop
• To swivel the brightfield front lens H into the
optical path, set lever (
4-117/4) in position I
(darkfield front lens D swiveled out). To swivel it
out, set lever in position 0.
• Before swiveling the darkfield front lens D in or
out, it is necessary to pull out the wide-field DF
slider (
4-117/5) from the universal condenser
(outer click-stop position). To swivel this lens in,
set lever (
4-117/7) in position 0. To swivel it out,
set the lever in position I.
• For immersion applications, screw the darkfield
attachment 1.2-1.4 Oil onto the darkfield front
lens and apply Immersol 518 F
®
immersion oil.
(5) Use of achromatic-aplanatic condenser 0.9 H/0.4 LD
Achromatic-aplanatic condenser 0.9 H/0.4 LD is equipped with a 0.9 front lens for brightfield use and a
second front lens (0.4) for brightfield and phase contrast applications, using slider Ph 1 or Ph 2 at a
working distance of approximately 14 mm
• The front lenses are swiveled in and out in the same manner as described for achromatic-aplanatic
universal condenser 0.9 H/0.8-0.9 DF.
• Slider Ph must be removed from the condenser when front lens 0.4 is swiveled in or out.
Fig. 4-117 Achromatic-aplanatic universal
condenser 0.9 H/0.8-0.9 DF
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