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Zeiss Axio Imager - Page 163

Zeiss Axio Imager
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OPERATION
Axio Imager Illumination and contrast methods Carl Zeiss
M70-2-0020 e 06/2009 430000-7344-001 163
Fig. 4-125 Components for circular polarization contrast
Do not view an (anisotropic) specimen unless the above adjustment has been done.
Place the specimen to be examined onto the stage.
The specimens appear constantly and independently of the stage rotation in their specific interference
color, which depends on the material, specimen thickness and orientation.
To obtain a contrasty image at high objective magnifications (from approx. 20x), reduce the
illumination aperture to a value of between 0.15 and 0.20, i.e. close the aperture diaphragm
accordingly.
The effect of the λ/4 plate (
4-125/2) can be undone by either swiveling it out of the light path
or turning it with lever (
4-125/1) into one of its two click-stop positions.

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