42 Agilent 1290 Infinity Binary Pump User Manual
4 Using the Pump
Preparing the Binary Pump
Preparing the Binary Pump
For best performance of the pump:
• Place solvent cabinet with the solvent bottles always on top (or at a higher
level) of the pump.
• For optimum performance, use the built-in degasser. This is mandatory for
flow rates below 0.5 mL/min and for configurations without Jet Weaver.
• When using the pump with vacuum degassing unit, flush the degassing unit
with at least 5 mL per channel before operating the pump, especially when
the pumping system had been turned off for a certain length of time (for
example, overnight) and volatile solvent mixtures are used in the channels.
• Prevent blocking of solvent inlet filters (never use the pump without solvent
inlet filters). Growth of algae should be avoided, see “Algae Growth in HPLC
Systems” on page 43.
• Check pump outlet filters and column frit in regular time intervals. A
blocked pump outlet filter can be identified by black, yellow or greenish
layers on its surface.
• Whenever possible use a minimum flow rate of 5 µL/min per solvent
channel to avoid crossflow of solvent into the unused pump channel.
• When using buffer solutions, flush the system with water before switching
it off.
• The optional seal wash function should be used when buffer solutions with
concentrations of 0.1 M or higher are being pumped for long periods of
time.
• Never leave an unused pump with water in a channel for an extended
period of time (2-3 days). Always flush with organic solvent or add 10 %
isopropanol to water.
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