Agilent Technologies Infinity Binary Pump VL 1290 User Manual

Agilent 1290 Infinity Binary Pump User Manual 67
How to Optimize the Performance of Your Module
5
How to Achieve Higher Resolution
• %B = change in fraction of solvent B during the gradient,
• S = constant (ca. 4-5 for small molecules).
This shows that k and hence resolution can be increased by having a shallower
gradient (2 to 5 %/min change is a guideline), higher flow rate and a smaller
volume column. This equation also shows how to speed up an existing gradient
– if the flow is doubled but the gradient time is halved, k
*
remains constant
and the separation looks the same but happens in half the time. Recently
published research has shown how a shorter STM column (at temperatures
above 40 °C) can generate higher peak capacity than a longer STM column by
virtue of running it faster. (Refer to Petersson et al., J.Sep.Sci, 31, 2346-2357,
2008, Maximizing peak capacity and separation speed in liquid
chromatography).

Agilent Technologies Infinity Binary Pump VL 1290 Specifications

General

Brand	Agilent Technologies
Model	Infinity Binary Pump VL 1290
Category	Laboratory Equipment
Language	English