11. Feline A, B and AB Blood Typing
•
Blood samples with a hematocrit of less than
4 %
or greater than
60 %
are not
recommended for use with the Heska Element COAG
™
Analyzer. Blood samples
outside this range may result in an instrument error or inaccurate test result.
11.5. Test Procedure
Before performing any tests, be sure that the Heska Element COAG
™
Analyzer has been
installed properly in accordance with the instructions in this guide. See
Chapter 3
for
details regarding how to set the Analyzer up correctly.
Verify that you have the necessary materials and equipment for performing a Feline A &
B Blood Typing test, see Section 11.3.
Step 1:
Remove a pouch with a test cartridge from the refrigerator and let the pouch
and cartridge reach room temperature
59 °F
to
86 °F
/
15
◦
C
to
30
◦
C
before
starting the test.
Step 2:
Draw blood from the patient into a tube coated with or containing EDTA as an
anticoagulant. The assay requires only 100uL of whole blood but the syring or
tube should be full to ensure the correct concentration of EDTA. If the blood
type is not to be determined immediately, nutrients such as CPDA should not
be added.
Step 3: Determine the hematocrit of the sample.
Step 4:
Remove the cartridge and pipette tip from the pouch and the pre-filled tube
with diluent from the kit box.
Step 5:
From the Home Screen, touch the
Feline
Blood Typing button to start the test.
Step 6:
When the message Please insert new cartridge
flashes on the screen, insert the Feline A & B
Blood Typing test cartridge completely into the
cartridge slot on the front of the Analyzer. The
cartridge must have the label facing upward
and the sample well sticking out of the Ana-
lyzer.
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