10. Canine DEA 1 Blood Typing
•
The accuracy of the test results is dependent on the quality of the blood sample.
The quality of the blood sample is dependent upon the blood sample collection, the
proper blood to EDTA ratio, and the proper introduction of the sample and diluent
into the sample well. Please observe all precautions cited in this manual and use
good blood sample collection techniques at all times.
•
A very limited number of canine patients exhibit auto-agglutination of varying
degrees due to serum factors that cause agglutination of the patient’s own red
cells. The Canine DEA 1 cartridge has two channels – a reference channel and
a test channel. If auto-agglutination occurs in the reference channel, this will be
indicated on the results screen. If auto-agglutination occurs the blood should not
be used for transfusion and the dog should only be transfused with blood from a
DEA 1 negative donor. This occurs in only a very small percentage of patients.
•
Being an absorption assay, the test accuracy is dependent on proper dilution of
the whole blood before adding the sample to the cartridge. Always measure the
hematocrit of the sample prior to testing and follow the on-screen dilution procedure.
•
Hemolyzed blood samples may cause false DEA 1 negative results because the
Analyzer is unable to distinguish RBC agglutinates from the plasma containing
hemoglobin. Always verify that the sample is not hemolyzed when measuring the
hematocrit. As a general guideline, the plasma must be clear and more yellow than
red. If the sample is severely hemolyzed, a new blood sample should be drawn.
•
Blood samples with a hematocrit of less than
10 %
or greater than
70 %
are not
recommended for use with the Heska Element COAG
™
Analyzer. Blood samples
outside this range may result in an instrument error or inaccurate test result.
10.5. Test Procedure
Verify that you have the necessary materials and equipment for performing a Canine
DEA 1 Blood Typing test, see Section 10.3.
Step 1:
Remove a pouch with a test cartridge from the refrigerator and let the pouch
and cartridge reach room temperature
59 /degree F
to
86 /degree F
/
15
◦
C
to
30
◦
C before starting the test.
Step 2:
Draw blood from the patient into a tube coated with or containing EDTA as an
anticoagulant. The assay requires only 100uL of whole blood but the syringe
or tube should be full to ensure the correct concentration of EDTA. If the blood
type is not to be determined immediately, nutrients such as CPDA should not
be added.
Step 3: Determine the hematocrit of the sample.
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