Jasco V-560 - Quantitative Analysis and Spectrum Measurement; Quantitative Analysis Introduction

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3. Quantitative Analysis and Spectrum Measurement
T his section describes quantitative analysis and spectrum m easurem ent. T he param eters are described
only briefly in order to clarify the operation flow . F ollow the procedures outlined below in order to becom e
fam iliar w ith the operation of V -500W . For m ore detailed inform ation, see the section for each program .
3.1 Q uantitative Analysis Introduction
T he follow ing sections briefly describe the quantitative analysis program and its operation flow , follow ed by
the procedure for creating calibration curves, unknow n sam ple m easurem ent, and saving and printing
results.
3.1.1 Q uantitative analysis program overview
3.1.1.1 Quantitative analysis program
T he quantitative analysis program has the follow ing features.
(1) T hree m ethods of analysis outlined.
1) 1-w avelength quantitative analysis. F ig. 3.1 (1).
U sed for norm al solution sam ple.
2) 2-w avelength quantitative analysis. F ig. 3.1 (2).
U sed for baseline correction.
3) 3-w avelength quantitative analysis. F ig. 3.1 (3).
U sed for baseline correction.
T he follow ing form ula is used for 3-w avelength quantitative analysis. W L1 is the w avelength and E
(1) the absorbance at that w avelength.
DAbs E
WL
WL
E
WL
WL
E
WL WL
= -
-
×
+
-
×
-
( )
|
|
(
)
|
|
(
)
| |
1
1
2
3
3
1
2
3 2
WL 1 WL 1WL 2
D Abs
D Abs
WL 1WL 2 WL 3
E(2)
E(1)
E(3)
(1) (2) (3)
1-wavelength
2-wavelength 3-wavelength
(Peak) (Base 1) (Peak) (Peak)(Base 1) (Base2)
F igure 3.1 Q uantitative analysis m ethods
(2) T he calibration curve can be selected from the m odes show n below , according to the application.