Recording Chlorophyll Fluorescence
CIRAS-2 Operator's Manual Version 2.04
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phiPSR Options
Note. ”Complete CFM Recording with”
must be checked.
After starting the measurement, the main CIRAS display will show the following:
In the upper left hand corner, you will see a message showing “CFM Working” with a timer showing the
status of the dark adaptation period. You can end the dark adaptation at any time by pressing the End
DA button.
During dark adaptation, the LED light unit is automatically turned off. During this period, you should
witness a fluorescence signal (green line) of 0 on the lower display and your photosynthesis calculations
corresponding to the actual response of the plant under set conditions. A positive value (+) corresponds
to photosynthesis and a negative value (-) corresponds to respiration. The reference CO
2
(Cr) and H
2
O
(Hr) should be stable with little differentials for CO
2
(Cd) and H
2
O (Hd) during dark adaptation.
At the completion of the dark adaptation period or when you manually end it, the Fv/FM measurement will
be initiated and recorded: