Observing and Recording Leaf Gas Exchange
CIRAS-2 Operator's Manual Version 2.04
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Go to Utilities – Stored Diff Bal. Enter the range of values and click OK.
The cuvette must be fully connected, though it does not have to be closed and a CO
2
cartridge must be
present. The measurement screen will then be displayed and initially CIRAS-2 will zero. CIRAS-2 will
then be set to the first set of values.
When the graph shows that the readings have stabilized click on Steady. CIRAS-2 will now do a Diff Bal
and on completion, set to the next pair of values. The process should repeat 5 more times. On
completion of the sixth pair, quadratic equations are fitted to the measured Reference concentrations and
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