Before cleaning the retort, make sure no tissues are left in the retort.
When the processing is complete, remove the tissues from the retort.
The tissues may be damaged if they remain in the retort after the processing has completed. Do not leave
the tissues for an extended period of time in the retort.
Check the reagent and paraffin volumes before commencing the tissue processing.
The solution volume will decrease through repeated tissue processing, so be sure to add reagent. If tissue
processing is performed when any reagent or paraffin is insufficient or not filled to the specified volume,
tissues may not be immersed in the reagent, resulting in improper processing of tissues. Also, be careful
not to fill too much reagent because it can cause instrument failure. If there is substantially less reagent in
any station (the station is empty) although the processing was successful, an error may have occurred
during the previous processing. Do not start the next operation, but contact the Sakura Finetek Technical
Support representative or local representative.
Do not put solid paraffin. Visually confirm that the paraffin has melted.
Use molten paraffin. If the instrument is operated when the paraffin is not molten, a failure may occur due to
clogged tubing, etc. If the melting point of paraffin is set to the preset temperature, the paraffin may not melt
completely depending on the environmental temperature.
If any reagent of low boiling point (methanol, ethanol, etc.) is to be used for long hours
of processing at high concentration, use the P/V [OFF] mode or heating [OFF] mode.
If kept under reduced pressure for a long period of time, the reagent may decrease in volume and cause
the tissues to get dry or shrink.
Operating the instrument with an inappropriate temperature setting, time setting
(long/short) or P/V setting may damage the tissues. Conduct a verification test
beforehand to confirm that tissue samples can be prepared properly. If you have any
question regarding tissue processing programs, contact the Sakura Finetek Technical
Support representative or local representative.
The O-ring at the paraffin container insertion part must be inspected periodically.
If the O-ring at the paraffin container insertion part is damaged, paraffin can no longer be filled and the
tissues may be damaged. Inspect the O-ring periodically.
Assigning a correct solution group is the basis of automatic transfer. If a wrong
solution group is assigned, secondary contamination of reagent or tissue processing
failure may occur.
If the reagent on the screen is different from the reagent actually set, the tissue processing will be adversely
affected. Exercise due caution when adding or changing reagent.
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