Table of Contents xv
Caution symbol .................................................................................................. A-5
Warnings that apply to all Waters instruments ......................................... A-5
Electrical and handling symbols ................................................................. A-13
Electrical symbols.......................................................................................... A-13
Handling symbols .......................................................................................... A-14
B Detector Specifications ....................................................................... B-1
Operational specifications ............................................................................. B-2
Optical specifications ..................................................................................... B-4
Optional Waters TaperSlit Flow Cell specifications ............................... B-5
C Spare Parts ............................................................................................ C-1
D Solvent Considerations ....................................................................... D-1
Introduction ...................................................................................................... D-2
Clean solvents.................................................................................................. D-2
Solvent quality................................................................................................. D-2
Preparation checklist....................................................................................... D-2
Water................................................................................................................ D-2
Using buffers.................................................................................................... D-2
Tetrahydrofuran .............................................................................................. D-3
Solvent miscibility ........................................................................................... D-3
How to use miscibility numbers...................................................................... D-5
Buffered solvents ............................................................................................. D-6
Head height ....................................................................................................... D-6
Solvent viscosity ............................................................................................... D-6
Mobile phase solvent degassing ................................................................... D-6
Gas solubility ................................................................................................... D-7
Effects of intermolecular forces .................................................................. D-7
Effects of temperature ................................................................................ D-7
Effects of partial pressure ........................................................................... D-7