D-10 Solvent Considerations
Mixed mobile phases
The table below contains approximate wavelength cutoffs for some other
solvents, buffers, detergents, and mobile phases. The solvent concentrations
represented are those most commonly used. If you want to use a different
concentration, you can determine approximate absorbance using Beer’s Law,
because absorbance is proportional to concentration.
Acetone 330 Isopropanol 205
Acetonitrile 190 Isopropyl chloride 225
Amyl alcohol 210 Isopropyl ether 220
Amyl chloride 225 Methanol 205
Benzene 280 Methyl acetate 260
Carbon disulfide 380 Methyl ethyl ketone 330
Carbon tetrachloride 265 Methyl isobutyl
ketone
334
Chloroform 245 Methylene chloride 233
Cyclohexane 200 n-Pentane 190
Cyclopentane 200 n-Propanol 210
Diethyl amine 275 n-Propyl chloride 225
Dioxane 215 Nitromethane 380
Ethanol 210 Petroleum ether 210
Ethyl acetate 256 Pyridine 330
Ethyl ether 220 Tetrahydrofuran 230
Ethyl sulfide 290 Toluene 285
Ethylene dichloride 230 Xylene 290
UV cutoff wavelengths for common chromatographic solvents
Solvent
UV Cutoff
(nm)
Solvent
UV Cutoff
(nm)
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