72 Operation Manual
6 Chromatographic Checkout
To Check uECD Performance
1 Gather the following:
• Evaluation column, HP- 5 30 m × 0.32 mm × 0.25 µm
(19091J- 413)
• uECD performance evaluation (checkout) sample
(18713–60040, Japan: 5183- 0379)
• 4- mL solvent and waste bottles or equivalent for
autoinjector.
• Chromatographic- grade isooctane
• 2- mL sample vials or equivalent for sample.
• Inlet and injector hardware (See “To Prepare for
Chromatographic Checkout.”)
2 Verify the following:
• Clean fused silica indented mixing liner installed. If
not, install it.
• Chromatographic- grade gases plumbed and configured:
helium for carrier gas, nitrogen for makeup.
• Empty waste vials loaded in sample turret.
• 4- mL vial with diffusion cap filled with hexane and
inserted in Solvent A injector position.
3 Replace consumable parts (liner, septum, traps, syringe,
and so forth) as needed for the checkout. See “To Prepare
for Chromatographic Checkout.”
4 Install the evaluation column. (See the procedure for the
SS, PP, COC, MMI, or PTV in the Maintenance manual.)
• Bake out the evaluation column for at least 30 minutes
at 180 °C. (See the procedure for the SS, PP, COC,
MMI, or PTV in the Maintenance manual.)
• Be sure to configure the column.
5 Display the signal output to determine baseline output. A
stable baseline output at any value between 0.5 and
1000 Hz (OpenLAB CDS ChemStation Edition display
units) (inclusive) is acceptable.
• If the baseline output is < 0.5 Hz, verify that the
electrometer is on. If the offset is still < 0.5 Hz, your
detector requires service.
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