Chromatographic Checkout 6
Operation Manual 57
To Check FID Performance
1 Gather the following:
• Evaluation column, HP- 5 30 m × 0.32 mm × 0.25 µm
(19091J- 413)
• FID performance evaluation (checkout) sample
(5188- 5372)
• Chromatographic- grade isooctane
• 4- mL solvent and waste bottles or equivalent for
autoinjector
• 2- mL sample vials or equivalent for sample
• Inlet and injector hardware (See “To Prepare for
Chromatographic Checkout.”)
2 Verify the following:
• Capillary column jet installed. If not, select and install
a capillary column jet.
• Capillary column adapter installed (adaptable FID
only). If not, install it.
• Chromatographic- grade gases plumbed and configured:
helium as carrier gas, nitrogen, hydrogen, and air.
• Empty waste vials loaded in sample turret.
• 4- mL solvent vial with diffusion cap filled with
isooctane and inserted in Solvent A injector position.
3 Replace consumable parts (liner, septum, traps, syringe,
and so forth) as needed for the checkout. See “To Prepare
for Chromatographic Checkout.”
4 Install the evaluation column. (See the procedure for the
SS, PP, COC, MMI, or PTV in the Maintenance manual.)
• Bake out the evaluation column for at least 30 min at
180 °C. (See the procedure for the SS, PP, COC, MMI,
or PTV in the Maintenance manual.)
• Be sure to configure the column.
5 Check the FID baseline output. The output should be
between 5 pA and 20 pA and relatively stable. (If using a
gas generator or ultra pure gas, the signal may stabilize
below 5 pA.) If the output is outside this range or
unstable, resolve this problem before continuing.
6 If the output is too low:
• Check that the electrometer is on.
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