Optimizing Performance 2
1100 Series WS MWS Reference Manual 47
Using an Injector Program
The process is based on a program that switches the bypass grove of the
injection valve into the flow line for cleaning. This switching event is
performed at the end of the equilibration time to ensure that the bypass grove
is filled with the start concentration of the mobile phase. Otherwise the
separation could be influenced, especially if microbore columns are used.
For example:
Outside wash of needle before injection: 14 sec. using flush port
Injector program:
Draw x.x (y) µl from sample
Needle wash as method
Inject
Wait (equilibration time - see text above)
Valve bypass
Wait 0.2 min
Valve mainpass
Valve bypass
Valve mainpass
Using such an injector program in addition with the needle wash in the flush
port can reduce the carry-over by a factor of about 10 compared with a
standard needle wash in the flush port only.
NOTE
The live time of the tubing in the peristaltic pump is shorted by the usage of organic
solvents.
NOTE
Overlapped injection together with additional injection valve switching is not possible.
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