50 1100 Series WS MWS Reference Manual
2 Optimizing Performance
The injection cycle times also depend on the injection volume. In identically
standard condition, injecting 100 µl instead of 1 µl, increase the injection time
by approximately 8 sec. In this case and if the viscosity of the sample allows it,
the draw and eject speed of the injection system has to be increased.
General Recommendations for Fast Injection Cycle Times
As described in this section, the first step to provide short cycle times are
optimizing the chromatographic conditions. If this is done the well-plate
sampler parameter should be set to:
• Overlapped injection mode
• Wash time for needle outside set to 10 seconds
• Increase of draw and eject speed for large injection volumes
• Add at last run a blank, if overlapped injection is used
To reduce the injection time, the detector balance has to be set to OFF.
NOTE
For the last injection of the sequence with overlapped injections it has to be considered
that for this run the injection valve is not switched as for the previous runs and
consequently the injector delay volume is not bypassed. This means the retention times are
prolonged for the last run. Especially at low flow rates this can lead to retention time
changes which are to big for the actual calibration table. To overcome this it is
recommended to add an additional “blank” injection as last injection to the sequence.
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