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Applied Biosystems 7300 - Page 169

Applied Biosystems 7300
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Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 143
Index
L
lamp
failure 34
replacing 117
status, checking 117
warning messages displayed when running 117
layout, instrument and computer 14
lifting guidelines 12
line conditioner, requirements 9
loading a plate 40
long-term shutdown 104
M
maintenance
miscellaneous 106
monthly 105
schedule 105
semi-annual 106
weekly 105
materials, checking 14
moving and lifting, safety xx
moving guidelines 12
moving parts, safety xxiv
moving the 7300/7500 system 115
moving the system 12
MSDSs
description xxi
obtaining xiii, xxi
N
NED
pure dye 69, 80, 82, 84
network, wireless, do not use 10
networking the 7300/7500 system 10
O
omitting wells from use 95
optical calibration. See 7500/7500 Fast system optical
calibration
outliers 95
overvoltage category (rating) xxiv
P
packing kit, contents 15, 16
performing
background calibration 52
instrument function test 30
optical calibration (7500/7500 Fast systems
only) 59
pure dye run 71
RNase P run 90
ROI calibration 38
physical hazard safety xxiv
planning the installation 5
plate document
7500/7500 Fast optical calibration 61
background 54
pure dye calibration 72
RNase P plate 92
plates
Fast, description 38
Fast, do not vortex 38
loading 40
location of notched corner 40
number of reuses 52
standard, description 38
unloading 46
power button, location 21
power cords, discarding those that do not apply for your
voltage 21
Power Line Regulator 9
power options, setting 137
powering off the instrument 102
powering on the instrument 28
preparing
background plate 52
pure dye plate, custom 127
pure dye plates 71
RNase P plate 90
ROI calibration plate 38
protective devices, electrical 9
pure dye calibration
about 69
analyzing data 77
creating the plate document 72
custom 127
materials required 68
performing 73
preparing pure dye plates 71
spectra, 7300 80
spectra, 7500 82
spectra, 7500 Fast 84
spectra, evaluating 80
time required 68
when to perform 68
pure dye plate
creating custom
127
running 73
pure dye spectra 79
pure dyes 69, 80, 82, 84
R
R2 value 96
radioactive waste, handling xxiii

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