BD LSR II - Page 198

198 BD LSR II User’s Guide
C
calcium flux
about 90
experiment 92
measuring 98
optimization 91
optimizing sample 96
Time parameter 91
calculating compensation 64
calf thymocyte nuclei (CTN)
preparing 79
resolution 86
running 86
Calibrite beads 123
capacity, waste container 31
CEN See chicken erythrocyte nuclei.
changing battery 108
chicken erythrocyte nuclei (CEN)
optimization 80
preparing 79
running 84
Class I laser product xviii
Coherent lasers 33
compensation
calculating 64
controls 60
theory 133
tubes, creating 59
values 53
components
instrument shown 26
optical bench 32
computer system, about 36
configuration maps 158
containers, sheath and waste 31, 44, 47
control panel, instrument 27
controls
compensation 59, 60
single-stained 54, 66
cord, damaged xix
covers, instrument 26
Create Compensation Controls 59
creating
analysis objects 70
compensation tubes 59
global worksheets 66
statistics view 70
CTN See calf thymocyte nuclei.
custom configurations 162
cuvette flow cell 196
CV, troubleshooting 122
Cy-Chrome, filter configuration for 166
D
DAPI
and DNA 79
cleaning after using 102
data
analyzing 66, 70
gating 70
recording 66, 68
calcium flux 98
optimization 88
debris
excessive 122
removing 60
Default global worksheet checkbox 56
delay, laser 196
detectors
photodiode 35
photomultiplier tubes (PMTs) 35
sensitivity 36
dichroic
filters 34
mirrors 34, 133
digital data 26
discriminating filters (DFs) 132
LSR2.book Page 198 Tuesday, April 25, 2006 3:34 PM