Index 199
DNA
experiment criteria 78
flow rate for analysis 126
QC Particles kit 79
using DAPI 79
verifying linearity 78
doublet discrimination 79, 84
droplet containment system 30
troubleshooting 116
DsRed, filter configuration for 165
E
electrical
requirements xiii
safety xix
electronics 136
emission duration 136
environmental requirements xiii
event rate
erratic 120, 121
high 119
low 120
zero 117, 118, 119
excessive debris 122
excitation wavelength 128
experiments
calcium flux 92
DNA linearity 78
immunophenotyping 66
placement in folders 57
sample optimization 57
extra filters and mirrors 162
F
FACSFlow solution 123
Falcon tubes 116
filters
bandpass 34
optical
bandpass 34, 131
changing 42
dichroic 34
discriminating 132
extra 162
longpass 34, 130
shortpass 34, 131
specifications 43
theory 129
sheath, replacing 110, 111
FITC and Stokes shift 128
fixed-alignment lasers 26
flow cell 126
draining 49
flow rate control buttons 28
fluid control buttons 28
fluidics 126
cleaning 102
description 28, 126
flow rate control 28
flushing system 105
priming 49
removing air bubbles 47
run mode 28
sample injection port (SIP) 29, 30
fluorescence 128
emission 136
fluorochromes 133
emission spectra 129
filter configurations and 156, 162
role in light emission 128
which measured 41
forward scatter (FSC) 32, 33, 127
FSC and SSC voltages, adjusting 60
FSC threshold, adjusting 60
LSR2.book Page 199 Tuesday, April 25, 2006 3:34 PM
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