cytiva NanoAssemblr Ignite - Page 60

Total flow rate
Th
e total flow rate specifies the sum of the flow rates of the C and R channels, before
the optional dilution. A higher total flow rate value results in faster mixing. In most
nanoprecipitation reactions, a higher total flow rate results in smaller particles up to a
limit determined by the chemistry.
The table below gives the range of values that can be input for the parameter Total
flow rate.
Instrument Total flow rate
Ignite
0.1 to 20 mL/min
Ignite+
0.1 to 200 mL/min
Flow rate limitations on Ignite+
Du
e to the physical limitations of syringes, certain flow rates within the Ignite+ param-
eter range are restricted on smaller syringes. The table below gives the maximum flow
rate for a single channel for each compatible syringe by supplier.
Syringe
si
ze
Maximum flow rate for a single channel (mL/min)
BD B Braun
Injekt
B Braun
Omnifix
Terumo Shinva
Ande
KDL WEGO KOVAX
1 mL 20.8 20.8 20.8 20.8 20.8 20.6 - 20.4
2 mL - 88.7 - - 75.6 75.2 76.1 -
2.5 mL - - - 76.3 - - - -
3 mL 69.5 - 89.6 75.5 - - - -
5 mL 135 147 146 159 140 142 148 -
10 mL 196 238 238 235 206 205 209 -
20 mL 342 381 379 385 - - - -
30 mL 435 435 435 - - - -
Note:
The chosen flow rate ratio impacts the required flow rate per channel for a
given total flow rate. For example, to perform a run with the following
parameters,
• 200 mL/min total flow rate,
• 3:1 flow rate ratio ,
• and BD syringes,
the minimum syringe sizes that can be used are:
• one 10 mL syringe (at 150 mL/min),
• and one 3 mL syringe (at 50 mL/min).
5 Preparation
5
.
1 Parameters
5.1.2 Mixing parameters
NanoAssemblr Ignite and Ignite+ Operating Instructions NIN1134 AA 60