cytiva NanoAssemblr Ignite - Waste Parameters

5.1.3 Waste parameters
In
troduction
The instrument can collect fluid at the beginning and the end of a procedure to
decrease the variation in the particle characteristics of the output.
At the beginning of a run, the fluid flow rate can vary from the target as the paddles
accelerate to full speed. Before steady-state flow has been reached, there can be off-
target mixing between the reagents. The amount of start waste to collect depends on
the following factors:
• The syringe size. Syringes can deform under the forces applied by the instrument.
Larger syringes deform more than smaller syringes and therefore take longer to
reach the intended flow rate.
• The target flow rate. Higher flow rates result in a higher volume of fluid output as
the system reaches its intended flow rate
• The sensitivity of the formulation to flow rate parameters. Some formulations
are more sensitive than others to deviations in flow rate.
• The final application for the particles. Observed differences in size can affect
biological endpoints.
The parameter Start waste defines the volume of fluid to collect in the waste collec-
tion tube at the beginning of the run.
The parameter End waste defines the volume of fluid to collect in the waste collection
tube at the end of the run.
Start waste recommendations for
NxGen and NxGen Dilution Cartridges
The value for the parameter Start waste can be between 0 mL and the value of Total
volume.
The recommended values for the parameter Start waste can be calculated by
summing the recommended start waste values for specific syringe sizes.
Note:
The following recommendations suggest collecting a larger amount of
wa
ste to ensure the most uniform population of particles. If it is desirable to
collect less waste, optimize the parameter values by systematically
collecting less waste and testing each result. Contact us for further help
with parameter optimization. See Contact information for support,
on page 119.
If using two different sized syringes in the C and R channels, add the values listed for
th
e smaller syringe size and the larger syringe. If using dilution, add the value from the
column Dilution syringe. If using same size syringes for both channels, skip to the
second table for calculation recommendations.
5 Preparation
5.
1 Parameters
5.1.3 Waste parameters
NanoAssemblr Ignite and Ignite+ Operating Instructions NIN1134 AA 62