cytiva NanoAssemblr Ignite - Page 63

Syringe size
(m
L)
Values used to calculate Start waste for different sized
syringes (mL)
Smaller syringe Larger syringe Dilution syringe
1 0.10 N/A 0.20
3 0.15 0.35 0.25
5 0.20 0.50 0.35
10 0.30 0.80 0.50
20 - - 0.65
30 - - 0.65
If the syringes for the C and R channels are the same size, use the start waste value
li
sted in the table below. If using dilution, add the value from the column Dilution
syringe. If using same size syringes for both channels, skip to the second table for
calculation recommendations.
Syringe size (mL)
Values used to calculate St
art waste for same sized
syringes (mL)
Same size syringes Dilution syringe
1 0.3 0.20
3 0.50 0.25
5 0.70 0.35
10 1.10 0.50
20 - 0.65
30 - 0.65
Start waste r
ecommendations for
NxGen 500 and NxGen 500D
Cartridges
The value for the parameter Start waste can be between 0 mL and the value of Total
volume.
The recommended values for the parameter Start waste can be calculated by
summing the recommended start waste values for specific syringe sizes.
Note:
The following recommendations suggest collecting a larger amount of
wa
ste to ensure the most uniform population of particles. If it is desirable to
collect less waste, optimize the parameter values by systematically
collecting less waste and testing each result. Contact us for further help
with parameter optimization. See Contact information for support,
on page 119.
5 Preparation
5.
1 Parameters
5.1.3 Waste parameters
NanoAssemblr Ignite and Ignite+ Operating Instructions NIN1134 AA 63