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Preparing the Biosafety Cabinet for Gaseous Sterilization
Note: This section only reviews the steps required for preparing the biosafety cabinet
for gas sterilization. Thoroughly understand the sterilization procedures and protocols
supplied by the manufacturer of the sterilizing system before attempting this operation.
1. Consult the sterilization equipment manufacturer to determine if the biosafety
cabinet’s internal blower(s) should be on or off during the sterilization process.
2. Thoroughly surface decontaminate the working area of the cabinet.
3. Remove the work surface after it has been surface decontaminated.
4. Remove the front grille.
5. Place the work surface and the grille into the work area for decontamination.
6. Remove the front dress panel.
7. Remove the sash stop bracket, located near the top of the blower plenum cover.
8. Lift the sash up until the bottom edge of the sash clears the bottom edge of the
Supply HEPA Filter shelf.
9. Using flexible plastic sheet 5-8 mil thick and duct tape, seal the perimeter of the
front opening. If you wish to connect the sterilizer’s discharge tube to the
biosafety cabinet’s Upstream Sampling/Decontamination Tube, it is located by
the towel catch.
10. Open the exhaust filter cover.
Note: DO NOT attempt to remove the brackets that secure the exhaust filter
cover; they are needed to maintain rigidity on the top of the chassis.
11. Using flexible plastic sheet and duct tape, seal the exhaust filter opening.
12. The biosafety cabinet is now prepared for gaseous decontamination.
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