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Lighting Test (optional)
The light intensity should be measured as described in the current NSF/ANSI Standard
49 Annex F. To test the light intensity at the biosafety cabinet’s work surface, follow
these instructions:
Note: A light meter that is color- and cosign-corrected must be used for accurate
results.
1. Mark test points on the work surface as follows:
a. Mark the front-to-back centerline of the work surface
b. Starting 6 inches (15.2 cm) from the left side wall, mark a test point on the
front-to-back centerline.
c. Continue marking additional test points along the centerline at 12 inch
(30.5 cm) increments until the left-to-right center of the work surface is
reached.
d. Repeat steps b and c starting from the right side wall.
e. All test points are now marked.
2. With the biosafety cabinet lights off, take a background reading with the light
meter. It should be 15 foot-candles (161 lux) or less.
3. Turn on the lights of the biosafety cabinet. Wait 1 minute.
4. Place the light meter sensor at each of the marked test points and take a
reading.
5. Average all test point readings.
Acceptance Criteria
The average light level shall be no less than 45 foot-candles (484 lux) greater than the
background light level reading.
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