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Noise Test (optional)
The noise (sound pressure) should be measured as described in the current NSF/ANSI
Standard 49 Annex F. To test the biosafety cabinet’s noise level, follow these
instructions:
1. Establish the sound meter location, as follows:
a. On the left-to-right centerline of the biosafety cabinet
b. 14 inches (35.6 cm) above the work surface
c. 12 inches (30.5 cm) from the front of the biosafety cabinet
2. With the biosafety cabinet’s blower(s) off, take a background reading with the
sound meter. It should be less than 55 dbA.
3. Start the blower(s) on the biosafety cabinet.
4. Place the sound meter at the location defined in Step 1, take a sound reading.
Acceptance Criteria
The noise (sound pressure) shall be no more than 67 dbA when the biosafety cabinet is
new, and no more than 73 dbA when the filters are loaded with particulate.
Vibration Test (optional)
The vibration level at the work surface should be measured as described in the current
NSF/ANSI Standard 49 Annex F. To test the biosafety cabinet’s work surface vibration
level, follow these instructions:
Note: The vibration meter must have a range of 20-20,000 Hz and provide an output in
displacement to proceed with this test.
1. Mark the geometric center of the work surface.
2. Place the vibration meter probe at this point, and with the biosafety cabinet’s
blower(s) off, take a background reading.
3. Start the biosafety cabinet’s blower(s). Wait 1 minute.
4. Take a reading at the same work surface location.
5. Subtract the reading with the blower(s) on from the background reading.
Acceptance Criteria
Net vibration shall be less than 0.0002 inches (5 x 10
-6
m) when the biosafety cabinet is
new.
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