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Sakura Histo-Tek VP1 User Manual

Sakura Histo-Tek VP1
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1. Troubleshooting
8-6
Instrument condition
Probable cause
Action
Paraffin overflowed.
Too much paraffin was filled to the
paraffin container.
If paraffin is added to above the top graduation in the paraffin
container, it may overflow as reagent is brought in, etc. It is necessary
to always confirm that paraffin is not above the upper limit of the
graduation before the start of processing.
Specimens and cassettes, etc., whose
brought in amount is large were
processed.
This problem may occur when a lot of specimens are processed
under conditions where more reagent is brought in, such as when
biopsy sheets, sponges or mesh cassettes are used. It is necessary
to come up with ideas to minimize the amount of reagent brought in,
or reduce the processing quantity.
Specimens are cold and the paraffin
processing time is short.
At Sta. 11 paraffin, processing is started in the condition where the
load such as specimens and baskets are cold, so paraffin may solidify
around the load. If the processing at that station ends in the condition
where the paraffin is still solid, a large amount of solid paraffin may be
brought into the next station. If the load is large or the reagent
temperature in Sta. 10 is low, sufficient processing time must be
ensured at the first paraffin station.
The paraffin container was refilled
while paraffin was still in the retort.
This problem occurs, for example, when paraffin is added to the
paraffin container at the last station while paraffin is still in the retort
after the end of tissue processing.
Places of the containers were
swapped during processing.
This problem occurs when an empty container after fill is swapped
with a container with solution.
Remedial action when
paraffin leaks outside
from the paraffin oven
If paraffin overflowed from the spill tray and attached to the interior or
door of the bottle rack, remove all bottles and scrape off paraffin
deposits attached in each area using a paraffin scraper, and then
wipe off any remaining paraffin using xylene, etc. Paraffin should be
attached to the top coupler unit, so remove the coupler unit and
remove the paraffin deposits. Scrape off the paraffin attached to the
reagent bottle using a paraffin scraper, and then wipe off any
remaining paraffin using xylene, etc.
Paraffin hardened in
the paraffin oven.
The ambient operating temperature is
too low.
Paraffin sometimes solidifies when the operating temperature of the
instrument drops to 10C or below. Do not install the instrument in a
place where the operating temperature may drop to 10C or below.
The preset processing temperature is
too low for the paraffin melting point.
Since the melting point of paraffin varies, presetting the paraffin oven
temperature to the melting point may cause the paraffin to solidify.
(Set the paraffin oven temperature automatically from the preset
processing temperature). Make sure the preset processing
temperature is at least 3C higher than the melting point.
A power outage occurred.
If power outage occurs, paraffin may solidify while the power is out.
Check to see if power outage occurred. If the paraffin solidified due to
power outage, it is necessary to wait until it melts.
Solid paraffin was added.
If solid paraffin is added to the paraffin container, temperature of the
entire oven may drop to cause paraffin solidification in the container.
Wait until it melts.
Paraffin oven door was kept open.
If the paraffin oven door is kept open, the temperature inside the oven
drops and paraffin may solidify. Do not keep the door open unless
necessary.
Activated carbon filter is not installed.
If the activated carbon filter is not set, exhaust from the oven
increases and more outside air is suctioned accordingly, so the
temperature inside the oven drops.
As a result, paraffin may solidify. In this case, set the activated carbon
filter.
The front side gets
dirty with paraffin.
Paraffin spilled when the paraffin
container was inserted/removed.
When inserting/removing the paraffin container, do so slowly to
prevent spilling of paraffin. Wipe off spilled paraffin immediately.
Paraffin spilled in the instrument when
the paraffin container was refilled or
moved.
When adding molten paraffin to the paraffin container, opening the
paraffin oven door and drawing out the container may dirty the
instrument if paraffin spills. To keep the instrument from getting dirty,
add paraffin or perform any other work involving paraffin outside the
instrument.
Too much paraffin in the paraffin
container, causing overflow.
If the paraffin container has too much paraffin, it may flow out of the
container during pumping-out.

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Sakura Histo-Tek VP1 Specifications

General IconGeneral
BrandSakura
ModelHisto-Tek VP1
CategoryLaboratory Equipment
LanguageEnglish

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