scinco Mega-800 - Measurement; Use Single Cell; Use Multi-Cells

25 Mega-800 SCINCO
3.5 Measurement
3.5.1 Use single cell
Single cell measurement includes fixed cell measurement and using one cell of multi-cell changer to
measure (refer to 3.3.6 single cell measurement).
First set up measurement parameters and sample cell parameters then perform auto zero. Put
sample into sample cell, then press key to measure its Abs or T%. Each time you press
key, it will remeasure the sample. The measurement results will be displayed as shown in
Fig. 3.13 (take Abs for example):
Fig. 3.13 Single cell measurement results
“No.‖ in the table refers to serial No. of the samples set by the user. ―2-1‖in the table refers to the
second measurement result of No.1 cell (if use multi-cell changer, it refers to current sample cell in
use); ―Abs‖ refers to absorbance value; ―K*Abs‖ refers to the value of measurement multiplied by K-
Factor. The K factor in the above table is 10.
3.5.2 Use multi-cells
First set up measurement parameters and sample cell parameters. The measurement procedures will
vary with the settings of No. 1 cell blank.
When Reagent Blank Correction in Cell No. 1 is set to “NO”
When Reagent Blank Correction in Cell No. 1 is set to ―NO‖, you need to perform autozero of cell
No.1. After autozero, put the samples into sample cells, then press key to start
measurement. And then the measured values will be calculated, saved and displayed on LCD one by
one.
When Reagent Blank Correction in Cell No. 1 (§3.5.3) is set to “YES”
When Reagent Blank Correction in Cell No. 1 is set to ―YES‖, you needn’t to perform autozero. The
system will first measure the set N samples. Then the measured values of samples No.2~No.N will be
deducted (corrected) by that of the blank in sample cell No. 1 following the formula below. The
corrected results will be recorded, calculated and displayed on LCD one by one.
 Absorbance:
Absorbance of Cell No. n= the measurement value of Cell No. n－Abs of Cell No.1 …………
( 3-1 )
 Transmittance:
The transmittance of sample No.n=measured value of sample No.n / measured value of
sample No.1(blank) × 100% …………（3-2）