scinco Mega-800 - Printout; Quit Measurement; Application Example

38 Mega-800 SCINCO
procedures:
First, press key to set sample module (5 cells or 8 cells);
Second, press key to move the cell to the preferred cell. (You also can press key to reset
cell;
Third, press key to return to the spectrum main menu, and then perform spectrum scanning
to the selected cell.
4.7 Printout
After measurement, you can press key to printout. Please refer to ―3.8 Printout‖.
If there are no measurement results, pressing key is inoperative.
4.8 Quit measurement
Press to quit measurement. It will prompt the user with the information ―Delete all the data?
(YES: Enter; NO: CE)‖. Press to return to the main menu of instrument.
4.9 Application example
Vitamin B12 has characteristic absorption peak at 361nm, which the operators always concern when
they perform spectrum scanning of vitamin B12. We will take vitamin B12 for example to show the
user how to perform spectrum scanning.
The procedures of using Mega-800 UV-VIS Spectrophotometer to measure the absorption peak of
Vitamin B12 are shown as follows:
① Mix standard vitamin B12 injection with deionized water in a proportion of 1:10 to get 10ml
dilution solution. Then pour the dilution solution and deionized water into two quartz cells
separately (deionized water as reference).
② Turn on MEGA-800 UV-VIS Spectrophotometer. After 60 minutes’ preheat time, press
to gain access to spectrum mode menu (refer to 4.1 gain access to spectrum mode).
③ Press key to gain access to parameters setting page. Set the photometric mode to
Abs by pressing key, set the scan speed to Middle by pressing key, set the
sample interval to 1nm by pressing , set the wavelength range to 220nm-660nm by
pressing , and set the ordinate range to 0.000-2.000 by pressing (please refer
to ―4.2 Parameters setting‖ for the detailed information).
④ Put the black block into the sample cell on the sample optical path (only this cell can be
used now). Press on the parameters setting page to perform dark current correction.
After correction, press to return to photometric mode menu.
Note:
Sometimes the spectrum displayed in the LCD is different from the printed one, because the resolution of
the printer is higher than that of the LCD. The spectrum data points displayed is less than that of the
prineted. But the peak values are the same.