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BD BACTEC FX40 - 4.5 Entering Vials

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4 – Operation
57
Collection
Specimens should be collected aseptically from the patient and inoculated into the vials. Refer to
the Media Package Insert for specific recommendations on specimen collection. Vials should be
labeled and sent to the laboratory at once.
Preparation
At least one aerobic culture vial and one anaerobic vial should be prepared. To prepare a vial,
remove the plastic flip cap and clean the exposed rubber septum with 70% isopropyl alcohol. Use
a separate swab for each vial. Inoculate the vial with the appropriate volume of sample (refer to the
Media Package Insert for specific information on vial inoculation).
4.5 Entering Vials
Collection - Platelets
Platelets should be collected aseptically from the bagged unit and inoculated into the vial. Refer to
the Media Package Insert for specific recommendations.
To enter vials, select an instrument where there are available stations. (The number of available
stations is shown below the vial entry icon on the Status display.)
Then follow one of the methods described below.
Vial Entry can be initiated in one of two ways:
Method 1 (Vial Activated)
Access the Status display (from any other display select the Status tab)
Select an instrument that has available stations, and open its door
The barcode scanner turns on
Scan a vial sequence barcode label
The Vial Entry display appears and the Sequence, Media, and default Protocol are
automatically entered
If you did not scan the Accession, scan or enter it now (sequence and accession can be
scanned in any order)
To change the protocol, select Modify, then select the Up Arrow to increase or Down
Arrow to decrease the protocol length
Place the vial into an available station (solid green indicator)
Method 2 (Icon Activated)
Select an instrument that has available stations, and open its door
Select the vial entry button on the Status display
The Vial Entry display (Figure 4-4) appears and the barcode scanner turns on
Scan the vial sequence barcode label
The Sequence, Media, and default Protocol are automatically entered
If you did not scan the Accession, scan or enter it now
To change the protocol, select Modify, then select the Up Arrow to increase or Down
Arrow to decrease the protocol length
Place the vial into an available station (solid green indicator)

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