BD BACTEC FX40 - 4.9 Removing Positive, Negative, and Ongoing Vials

BD BACTEC™ FX40 Instrument User’s Manual
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To print a report:
1 Sele
ct the Reports tab.
2 Highlight the desired report by selecting it in the menu.
3 Select the desired criteria (Time Range, Sort By, Report By).
4 Select Print.
Refer to Section 5.4 for additional information and sample reports.
4.9 Removing Positive, Negative, and Ongoing Vials
Positive and Negative Vials
Many positive cultures will be detec
ted in the first 24 hours after inoculation. However, ongoing vials
must still be kept for several days to insure maximum recovery. With the BD BACTEC™ FX40
in
strument, vials are typically held for 5 days (except Myco/F Lytic and Mycosis IC/F Platelet
Aerobic/F, and Platelet Anaerobic/F vials) before they are discarded as negative. Each laboratory
should set the protocol length based on its own policies and conditions. Protocol lengths other than
5 days have not been evaluated.
You should perform a subculture and a Gram stain from
each positive vial. In most cases, organisms
can be identified and a preliminary report can be made to the physician. Preliminary antimicrobial
susceptibility (AST) and identification (ID) procedures may also be set up from fluid in the culture
vials. Many positive cultures will be detected in the first 24 hours after inoculation. However, ongoing
vials must still be kept for several days to ensure maximum recovery. With the BD BACTEC™ FX
instrument, vials are typically held for 5 days (except Myco/F Lytic, Mycosis IC/F, Platelet Aerobic/F,
and Platelet Anaerobic/F vials) before they are discarded as negative.
In a BD EpiCenter™ configuration, you can remove
Negative and Positive (but not related) vials
from an instrument in degraded mode.
Single Negative Vial Removal vs. Batch Vial Removal
The instrument can be configured for either single n
egative vial removal or batch negative removal.
This option is set up in the Configuration – Lab display.
For Single Vial Removal, each negative vial that is re
moved must be scanned to confirm its removal.
For Batch Vial Removal, vials do not have to be scanned. Vial presence sensors immediately sense
th
e removal of a vial and update the station LED indication and the status shown on the LCD.
Vial Reentry
NOTE
For optimal time to detection and recovery, it is recommended that
via
ls remain in their original station throughout the protocol. See
table below.