ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
4.7
GYNECOLOGIC SAMPLE PREPARATION
4 a. Carefully pour off the supernatant from the centrifuge tube to
avoid loss of cells. Dispose of according to local regulations.
b. Vortex the centrifuge tube briefly.
c. Pour 30 mL of the CytoLyt
™
Solution and 10% glacial acetic acid
mixture into the centrifuge tube and cap securely.
d. Invert the centrifuge tube by hand several times to mix.
5 Pellet the cells again by centrifugation - 1200 x
g
for 5 minutes.
6 a. Carefully pour off the supernatant from the centrifuge tube to
avoid loss of cells. Dispose of according to local regulations.
b. Vortex the centrifuge tube briefly.
7 a. Using the volume markings on the centrifuge tube, pour the
necessary quantity of unused (i.e., containing no patient specimens)
PreservCyt
™
Solution to the cells and fill to a final volume of 20 mL.
Secure the cap tightly.
b. Invert the centrifuge tube several times to mix and transfer the
sample back into the retained specimen vial.
8
Process the specimen using a ThinPrep
™
5000 processor with
AutoLoader according to the procedure for running gynecologic
specimens. Evaluate the resultant slide according to
The Bethesda
System for Reporting Cervical Cytology
. If after reprocessing, negative
results from specimen do not fit with the clinical impression, a new
specimen may be necessary.
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