ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
5.5
NON-GYNECOLOGIC SAMPLE PREPARATION
Refer to page 5.12 for CytoLyt Solution wash instructions.
Note:
See Chapter 3, PreservCyt™ & CytoLyt™ Solutions, for more information on CytoLyt
Solution.
WARNING:
CytoLyt Solution is a poison (contains methanol) and it must never come in direct
contact with the patient.
GENERAL STEPS FOR SAMPLE PREPARATION
CONCENTRATE BY CENTRIFUGATION – 600g for 10 minutes
The purpose of this procedure is to concentrate the cellular material in order to
separate the cellular component(s) from the supernatant. This step is performed
with fresh samples and after the addition of CytoLyt
™
Solution. When specified
in the protocol, centrifuge samples at 600 times normal gravity (600g) for
10 minutes to force the cells in solution into a pellet at the bottom of the
centrifuge tube.
Set your centrifuge to the approximate number of revolutions per minute (rpm)
to spin the cells at 600g.
Follow these steps to determine the correct setting for your centrifuge:
CAUTION:
Check cell morphology on non-critical experimental samples before making any
changes to your centrifugation process.
Note:
Use of fixed-angle centrifuges is not recommended.
Measure the rotor length of your centrifuge.
Use a centimeter ruler to measure the radius of your centrifuge, the distance from the center of the
rotor to the bottom of the bucket extended horizontally as shown in Figure 5-1.
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