NON-GYNECOLOGIC SAMPLE PREPARATION
5.12
ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
CYTOLYT SOLUTION WASH
Addition of CytoLyt
™
Solution to cell pellets is required to wash the sample. A
CytoLyt Solution
Wash
performs the following functions while preserving cellular morphology:
• Lyse red blood cells
• Dissolve mucus
• Reduce protein precipitation
A
CytoLyt Solution Wash
consists of the following process:
• Adding 30 mL of CytoLyt Solution to a cell pellet
•
Mucoid Specimens Only: Mechanical agitation
• Concentration by centrifugation — 600g x 10 minutes
• Pouring off the supernatant and vortexing to resuspend the cell pellet
One
CytoLyt Solution Wash
is usually adequate to clean most non-gyn samples. For particularly
bloody or mucoid specimens, additional
CytoLyt Solution Washes
may be necessary.
When a sample is collected in CytoLyt Solution at a ratio less than 30 parts CytoLyt Solution to 1 part
sample, this is considered a
Collection Step
and not a
Wash Step
. For example, if one collects 15 mL of a
sample and adds 30 mL of CytoLyt Solution to this sample, then the CytoLyt Solution: sample ratio is
only 2 to 1 and this is considered a sample collection step and still requires a
CytoLyt Solution Wash
.
For more information on CytoLyt Solution, refer to Chapter 3, PreservCyt™ & CytoLyt™ Solutions.
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