GYNECOLOGIC SAMPLE PREPARATION
4.6
ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
Dispose of PreservCyt Solution according to your guidelines for disposing of hazardous waste.
PreservCyt Solution contains methanol.
SAMPLE PROCESSING TROUBLESHOOTING
REPROCESSING A THINPREP
™
PAP TEST SAMPLE VIAL FOLLOWING AN UNSATISFACTORY
RESULT
Laboratory personnel may reprocess ThinPrep
™
Pap test specimens where slides have been
interpreted as inadequate (“Unsatisfactory for Evaluation”) for diagnosis following cytotechnologist
screening. The instructions below must be followed in order to properly reprocess these specimens:
Note:
Reprocessing a ThinPrep Pap test specimen may only be performed once.
Note:
Good laboratory practices should be followed to avoid introducing contaminants into the
PreservCyt Solution sample vial.
Reprocessing Protocol
1 Prepare a wash solution of sufficient volume to add 30 mL to every
ThinPrep Pap test specimen being reprocessed. The wash solution
is made by mixing 9 parts CytoLyt
™
Solution with 1 part glacial
acetic acid.
2 Prior to performing this step, assure there is sufficient volume in the
ThinPrep Pap test specimen to result in a pellet, following
centrifugation. Pour the contents of the ThinPrep Pap test specimen
into a centrifuge tube appropriately labeled to maintain chain of
custody. Retain the vial.
3 Pellet the contents of the centrifuge tube by centrifugation at
1200 x
g
for 5 minutes.
Note:
Once centrifugation is complete, the cell pellet should be
clearly visible but the cells may not be tightly packed
together (the pellet may appear fluffy).
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