Hologic ThinPrep 5000 - Page 111

NON-GYNECOLOGIC SAMPLE PREPARATION
5.4 ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
5
than one centrifuge tube of sample is used, the cell pellets can be combined after pouring off the
supernatant.
Other Sample Types
For non-mucoid brushings and scrapings that are received in PreservCyt
™
Solution, the sample is
ready to be run on the ThinPrep
™
5000 processor with AutoLoader.
For non-mucoid brushing and scrapings that are received in CytoLyt Solution, follow the protocol for
FNA samples. See page 5.14.
Urine Sample for use with the Vysis
®
UroVysion Assay
Do not exceed a 2:1 ratio of urine to PreservCyt Solution. If the urine volume exceeds 60 mL,
pour off excess. A minimum volume of 33 mL of urine is required to perform the Vysis
®
UroVysion assay.
Other Collection Media
In cases where CytoLyt Solution is contraindicated, balanced electrolyte solutions, such as
Plasma-Lyte and Polysol, may be used as collection media for samples to be processed on the
ThinPrep 5000 processor with AutoLoader. These solutions are primarily used as media for
washings or lavages which contact the patient.
Non-Recommended Collection Media
Hologic does not recommend the use of the following collection solutions with the ThinPrep system.
Use of these solutions will produce sub-optimal results:
• Sacomanno and other solutions containing carbowax
• Alcohol
• Mucollexx
®
• Normal Saline
• Culture media, RPMI Solution
• PBS
• Solutions containing formalin
Specimens
must be centrifuged and washed in CytoLyt
™
Solution and transferred to PreservCyt
™
Solution prior to being processed on the ThinPrep
™
5000 processor with AutoLoader.