ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
5.3
NON-GYNECOLOGIC SAMPLE PREPARATION
SPECIMEN COLLECTION
Note:
The ThinPrep
™
5000 processor with AutoLoader is designed for use with PreservCyt
™
Solution only. Do not use any other collection or preservative solution with the processor.
Samples to be processed on the ThinPrep processor with AutoLoader will arrive in the lab either
fresh or in CytoLyt
™
Solution. There are preferred collection methods for different sample types.
This section will describe the Hologic recommended procedure as well as alternate collection
methods.
WARNING:
For washes and lavages, do not expose the patient to CytoLyt Solution.
Fine Needle Aspirate Specimens
The optimal collection technique for FNAs is to deposit and rinse the entire sample into a centrifuge
tube containing 30 mL of CytoLyt Solution. A secondary method would be to collect the sample into
a balanced electrolyte solution, such as Polysol
®
or Plasma-Lyte
®
injection solutions.
Note:
Direct smears may be necessary for radiologic-guided FNAs when a rapid analysis of
specimen adequacy is required.
Mucoid Specimens
Mucoid specimens are best collected into CytoLyt Solution. If they are collected fresh, CytoLyt
Solution should be added as soon as possible. Early addition of CytoLyt Solution preserves the
sample and initiates the mucus dissolution process.
Large volume of fresh mucoid specimens (greater than 20 mL) should be concentrated before
addition of CytoLyt Solution to the sample.
Fluid Specimens
The preferred method for preparing fluid samples (urinary tract, effusions, synovial, and cyst fluids)
is to concentrate the fresh sample before any addition of CytoLyt Solution. If this is not possible and
the samples must be preserved for transport to the lab, collect the samples in CytoLyt Solution.
Note:
CytoLyt Solution added directly to fluids with high levels of protein may produce some
degree of protein precipitation.
Note:
Fluid collection in CytoLyt
™
Solution is only considered a Collection Step and not a Wash
Step. See page 5.12, CYTOLYT SOLUTION WASH, in this section for more detail.
The quantity of fluid samples can vary widely from less than 1 mL to 1000 mL and more. Each lab
must follow its own procedure for determining the amount of sample to use for processing. If more
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