NON-GYNECOLOGIC SAMPLE PREPARATION
5.14
ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
FINE NEEDLE ASPIRATES (FNA)
1. Collection:
Collect sample directly into 30 mL of CytoLyt
™
Solution. If
specimen must be collected in an intravenous solution, use a balanced
electrolyte solution.
Note
: If possible, flush the needle and syringe with a sterile
anticoagulant solution prior to aspirating the sample. Some
anticoagulants may interfere with other cell processing
techniques, so use caution if you plan to use the specimen for
other testing.
2. Concentrate by centrifugation — 600g for 10 minutes (page 5.5) or
1200 g for 5 minutes.
3. Pour off supernatant and vortex to resuspend cell pellet (page 5.7)
4. Evaluate cell pellet appearance (page 5.9)
If cell pellet is not free of blood, add 30 mL of CytoLyt Solution to cell
pellet and repeat from step 2.
5. Add appropriate amount of specimen (dependent on the size of the
cell pellet) to PreservCyt
™
Solution vial (page 5.10)
6. Allow to stand in PreservCyt Solution for 15 minutes (page 5.11)
7. Run on ThinPrep
™
5000 processor with AutoLoader using
Sequence
Non-Gyn.
Fix, stain, and evaluate.
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