ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
5.7
NON-GYNECOLOGIC SAMPLE PREPARATION
Figure 5-2 Determining the Correct Centrifuge Speed
To reduce the time required for the centrifugation step, operate your centrifuge at 1200g for 5 minutes.
POUR OFF SUPERNATANT AND VORTEX TO RESUSPEND CELL PELLET
Pour off the supernatant completely to effectively concentrate the sample. To do
this, invert the centrifuge tube 180 degrees in one smooth movement, pour off all
the supernatant, and then return the tube to its original position as shown in
Figure 5-3.
1
Observe the cell pellet during inversion to avoid accidental loss of
cellular material.
1. Refer to Bales, CE, and Durfee, GR. Cytologic Techniques in Koss, L. ed. Diagnostic Cytology and its
Histopathologic Basis. 3rd Edition. Philadelphia: JB Lippincott. Vol. II: pp. 1187–12600 for details.
Rotating radius (inches)
Rotating radius (centimeters)
Relative centrifugal force (gravities)
Speed - revolutions per minute
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