NON-GYNECOLOGIC SAMPLE PREPARATION
5.8
ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
CAUTION:
Failure to completely pour off the supernatant may produce a sparse sample and an
unsatisfactory slide due to dilution of the cell pellet.
Figure 5-3 Pouring Off Supernatant
After pouring off the supernatant, place the centrifuge tube onto a vortexor and agitate the cell
pellet for 3 seconds. Manual vortexing may be achieved by syringing the pellet back and forth
with a plastic pipette. The intention of this vortexing step is to randomize the cell pellet before
transferring to the PreservCyt
™
Solution vial and to improve the results of the CytoLyt
™
Solution
washing procedure.
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